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Am J Transl Res 2013;5(5):521-529
Original Article
A simplified two-stitch sleeve technique for arterial anastomosis of
cervical heterotopic cardiac transplantation in mice
Jing Fang, Long He, Shi-Qiang Wang, Ming-Jia Ma, Hong-Yun Liu, Xue-Hai Zhu, Ping Zhu, Xiang Wei, Cong-Yi
Wang
The Center for Biomedical Research; Key Laboratory of Organ Transplantation, Ministry of Education, Ministry of
Health; Department of Cardiovascular Surgery; Department of Medical Ultrasound, Tongji Hospital, Tongji Medical
College, Huazhong University of Science and Technology, Wuhan 430030, China; Guangdong General Hospital,
Guangdong Academy of Medical Sciences, Guangdong Cardiovascular Institute, Guangzhou 510080, China
Received July 27, 2013; Accepted August 11, 2013; Epub August 15, 2013; Published August 30, 2013
Abstract: Although cervical cardiac transplantation is a well recognized useful model in diverse experimental
settings, its widespread use, however, has been significantly hampered by the technical challenges relevant to
small vessel anastomosis. We herein introduced a simplified two-stitch sleeve technique into arterial
anastomosis during the course of cervical cardiac transplantation in mice. Cervical transplantation of allogenic
and syngeneic cardiac grafts was conducted to assess the feasibility of this two-stitch sleeve technique in arterial
anastomosis. Venous anastomosis was completed by the one-suture end-to-end microsuture technique, while
arterial anastomosis was conducted by invaginating the recipient right common carotid artery into the graft left
common carotid artery along with two guiding stitches. The two-stitch sleeve technique significantly simplified the
procedures for arterial anastomosis as compared with that of the traditional microsuture technique (5.5 ± 1.8 min
vs. 15.7 ± 3.0 min). However, the survival time for allografts (8.0 ± 0.2 day vs. 8.0 ± 0.4 day) and the long-term
patency for syngeneic grafts (> 120 days) were the same as the grafts implanted by the traditional microsuture
technique. This simplified sleeve technique is easy to learn, particularly for beginners without microsuture
experience, and therefore, it has the great potential for widespread use in transplant immunology.
(AJTR1307005).
Keywords: Anastomosis, heart transplantation, microsuture, two-stitch sleeve technique
Address correspondence to: Dr. Cong-Yi Wang, The Center for Biomedical Research, Tongji Hospital, Tongji
Medical College, Huazhong University of Science and Technology, 1095 Jiefang Ave., Wuhan, 430030, China.
E-mail: wangcy@tjh.tjmu.edu.cn; Dr. Xiang Wei, Department of Cardiovascular Surgery, Tongji Hospital, Wuhan
430030, China. E-mail: xiangwee@126.com
Original Article
A simplified two-stitch sleeve technique for arterial anastomosis of
cervical heterotopic cardiac transplantation in mice
Jing Fang, Long He, Shi-Qiang Wang, Ming-Jia Ma, Hong-Yun Liu, Xue-Hai Zhu, Ping Zhu, Xiang Wei, Cong-Yi
Wang
The Center for Biomedical Research; Key Laboratory of Organ Transplantation, Ministry of Education, Ministry of
Health; Department of Cardiovascular Surgery; Department of Medical Ultrasound, Tongji Hospital, Tongji Medical
College, Huazhong University of Science and Technology, Wuhan 430030, China; Guangdong General Hospital,
Guangdong Academy of Medical Sciences, Guangdong Cardiovascular Institute, Guangzhou 510080, China
Received July 27, 2013; Accepted August 11, 2013; Epub August 15, 2013; Published August 30, 2013
Abstract: Although cervical cardiac transplantation is a well recognized useful model in diverse experimental
settings, its widespread use, however, has been significantly hampered by the technical challenges relevant to
small vessel anastomosis. We herein introduced a simplified two-stitch sleeve technique into arterial
anastomosis during the course of cervical cardiac transplantation in mice. Cervical transplantation of allogenic
and syngeneic cardiac grafts was conducted to assess the feasibility of this two-stitch sleeve technique in arterial
anastomosis. Venous anastomosis was completed by the one-suture end-to-end microsuture technique, while
arterial anastomosis was conducted by invaginating the recipient right common carotid artery into the graft left
common carotid artery along with two guiding stitches. The two-stitch sleeve technique significantly simplified the
procedures for arterial anastomosis as compared with that of the traditional microsuture technique (5.5 ± 1.8 min
vs. 15.7 ± 3.0 min). However, the survival time for allografts (8.0 ± 0.2 day vs. 8.0 ± 0.4 day) and the long-term
patency for syngeneic grafts (> 120 days) were the same as the grafts implanted by the traditional microsuture
technique. This simplified sleeve technique is easy to learn, particularly for beginners without microsuture
experience, and therefore, it has the great potential for widespread use in transplant immunology.
(AJTR1307005).
Keywords: Anastomosis, heart transplantation, microsuture, two-stitch sleeve technique
Address correspondence to: Dr. Cong-Yi Wang, The Center for Biomedical Research, Tongji Hospital, Tongji
Medical College, Huazhong University of Science and Technology, 1095 Jiefang Ave., Wuhan, 430030, China.
E-mail: wangcy@tjh.tjmu.edu.cn; Dr. Xiang Wei, Department of Cardiovascular Surgery, Tongji Hospital, Wuhan
430030, China. E-mail: xiangwee@126.com
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