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Am J Translational Res 2011;3(2):139-148
Original Article
Proteomic identification of biomarkers of vascular injury
Ngan F. Huang, Kyle Kurpinski, Qizhi Fang, Randall J. Lee, Song Li
University of California San Francisco and University of California Berkeley Joint Program in Bioengineering,
Berkeley, CA, USA; Department of Medicine and Cardiovascular Research Institute, University of California San
Francisco, San Francisco, CA, USA; Department of Bioengineering, University of California Berkeley, Berkeley, CA,
USA; Division of Cardiovascular Medicine, Stanford University, Stanford, CA 94305, USA
Received November 8, 2010; accepted November 20, 2010; Epub November 21, 2010; published January 1, 2011
Abstract: Predictive biomarkers may be beneficial for detecting, diagnosing, and assessing the risk of restenosis
and vascular injury. We utilized proteomic profiling to identify protein markers in the blood following vascular
injury, and corroborated the differential protein expression with immunological approaches. Rats underwent
carotid artery injury, and plasma was collected after 2 or 5 weeks. Proteomic profiling was carried out by
two-dimensional differential in-gel electrophoresis. The differentially expressed plasma proteins were identified
by mass spectroscopy and confirmed by immunoblotting. Proteomic profiling by two-dimensional differential
in-gel electrophoresis and mass spectroscopy revealed plasma proteins that were differentially expressed 2
weeks after injury. Among the proteins identified included vitamin D binding protein (VDBP), aldolase A (aldo A),
and apolipoproteinE (apoE). Immunoblotting results validated a significant reduction in these proteins in the
plasma at 2 or 5 weeks after vascular injury, in comparison to control animals without vascular injury. These
findings suggest that VDBP, aldo A, and apoE may be biomarkers for vascular injury, which will have important
prognostic and diagnostic implications. (AJTR1011001).
Keywords: Vascular injury, angioplasty, apolipoprotein E, atherosclerosis, plasma marker, vitamin D binding
protein, proteomic profiling, aldolase
Full Text PDF
Address all correspondence to:
Song Li, PhD
University of California Berkeley
Department of Bioengineering
Berkeley, CA 94720-1762
Tel: (510) 666-2799
Fax: (510) 666-3381
Email: song_li@berkeley.edu
Original Article
Proteomic identification of biomarkers of vascular injury
Ngan F. Huang, Kyle Kurpinski, Qizhi Fang, Randall J. Lee, Song Li
University of California San Francisco and University of California Berkeley Joint Program in Bioengineering,
Berkeley, CA, USA; Department of Medicine and Cardiovascular Research Institute, University of California San
Francisco, San Francisco, CA, USA; Department of Bioengineering, University of California Berkeley, Berkeley, CA,
USA; Division of Cardiovascular Medicine, Stanford University, Stanford, CA 94305, USA
Received November 8, 2010; accepted November 20, 2010; Epub November 21, 2010; published January 1, 2011
Abstract: Predictive biomarkers may be beneficial for detecting, diagnosing, and assessing the risk of restenosis
and vascular injury. We utilized proteomic profiling to identify protein markers in the blood following vascular
injury, and corroborated the differential protein expression with immunological approaches. Rats underwent
carotid artery injury, and plasma was collected after 2 or 5 weeks. Proteomic profiling was carried out by
two-dimensional differential in-gel electrophoresis. The differentially expressed plasma proteins were identified
by mass spectroscopy and confirmed by immunoblotting. Proteomic profiling by two-dimensional differential
in-gel electrophoresis and mass spectroscopy revealed plasma proteins that were differentially expressed 2
weeks after injury. Among the proteins identified included vitamin D binding protein (VDBP), aldolase A (aldo A),
and apolipoproteinE (apoE). Immunoblotting results validated a significant reduction in these proteins in the
plasma at 2 or 5 weeks after vascular injury, in comparison to control animals without vascular injury. These
findings suggest that VDBP, aldo A, and apoE may be biomarkers for vascular injury, which will have important
prognostic and diagnostic implications. (AJTR1011001).
Keywords: Vascular injury, angioplasty, apolipoprotein E, atherosclerosis, plasma marker, vitamin D binding
protein, proteomic profiling, aldolase
Full Text PDF
Address all correspondence to:
Song Li, PhD
University of California Berkeley
Department of Bioengineering
Berkeley, CA 94720-1762
Tel: (510) 666-2799
Fax: (510) 666-3381
Email: song_li@berkeley.edu
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